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David Odde, Ph.D., IEM Cellular and Molecular Bioengineering Theme Co-Chair and Professor of Biomedical Engineering, is a Co-Principal Investigator on a recently awarded R01 grant from the National Institute on Aging. In collaboration with Dr. Elizabeth Rhoades (Principal Investigator, U. of Pennsylvania, Dept. of Chemistry) and Dr. Jonathan Sachs (Co-Investigator, U. of Minnesota Dept. of Biomedical Engineering), the $2.3 million 5-year grant will fund research into the molecular mechanisms of tau, a protein involved in Alzheimer's disease.
One of the hallmarks of Alzheimer's disease is the loss of function of the microtubule-associated protein tau, which leads to microtubule destabilization and disruption of normal neuronal function. One possible mechanism is that disease-specific modifications to the tau protein disrupt motor-based protein trafficking along axons through altered microtubule organization and dynamics. The goal of this collaborative project is to develop a molecular level understanding of tau's mechanism of action on microtubule assembly dynamics in both normal and disease-specific tau variants that affect tau-microtubule interactions. Using computational modeling, in vitro experimental analysis of purified proteins, and imaging microtubules in living cells, this project will develop a detailed biophysical mechanism of tau function towards the goal of being able to predict changes in cellular function from molecular-scale modifications to the tau protein. Results from this research will guide future therapy development to restore normal tau function in patients with Alzehimer's disease.
Khoa Cao completed his Master of Science degree in biomedical engineering in July 2016 for his project titled "Estimating mechanical differences between dorsal and ventral surfaces of U251 cells." Thanks for the hard work, Khoa!
The University of Minnesota was host to the 2nd Annual Midwest Tumor Microenvironment Meeting on May 18-20, 2016. Andrew, Ghaidan, and Louis presented posters, and the meeting was a great way to engage with other researchers within the very active local community. Special thanks to Paolo Provenzano, David Wood, Kaylee Schwertfeger, and James McCarthy for organizing!
The 3rd Minnesota Neuro-Oncology Symposium was held at the Hyatt Regency Hotel in downtown Minneapolis on May 9-10th, 2016. Andrew and Ghaidan both presented posters at the poster session on May 9th. Thanks to all attendees, especially the great set of speakers!
Biomedical engineering seniors Jesse Kasim and Matthew Hunt defended their honors theses in May 2016 and graduated with degrees in biomedical engineering. Next year, Jesse Kasim will be attending the University of Wisconsin Medical School, while Matthew Hunt will be working in R&D at Boston Scientific while he applies to medical school. Great job, and congratulations to the class of 2016!
Emily Tubman defended her Ph.D. thesis titled "Modeling and analysis of chromosome attachment error correction and congression during mitosis" in April 2016! She is currently working in Scientific Affairs at Boston Scientific as a Project Manager. Great job, Emily!
Rebecca Markovitz (Klank) defended her Ph.D. thesis titled "Physical determinants of glioma cell migration and disease progression" in September 2015! She is currently residing in Leipzig, Germany. Gut gemacht, Becky!
Graduate student Louis Prahl received a STEM Chateaubriand Fellowship and NSF Graduate Research Opportunities Worldwide (GROW) grant to study in France! He will spend 3 months in Paris, collaborating with the laboratory of Dr. Matthieu Piel at Institut Curie and learning microfabrication techniques to study cell migration. Tres bien, Louis!
We have two new papers out this month! First, former undergraduate Kelsey McCoy's paper Physical limits on kinesin-5 mediated chromosome congression in the smallest mitotic spindles was accepted to Molecular Biology of the Cell. This research, in collaboration with Judy Berman's laboratory (UMN/Tel Aviv University) and the Boulder Laboratory for 3D Electron Microscopy of Cells (University of Colorado) explores chromosome congression in the fungal yeast Candida Albicans. Next, former Master of Science student Joe Powers' paper The predicted role of steric specificity in crowding-mediated effects on reversible biomolecular association was accepted to Physical Biology, and will be in press later this year! Congratulations, all!
Graduate student Louis Prahl presented a poster titled "Increased traction force and reduced migration following microtubule depolymerization" at the EMBO Physics of Cells: From Molecules to Systems (PhysCell 2015). The third conference in its series, PhysCell 2015 was held at Banz Abbey, near Bad Staffelstein, Germany.
The Odde laboratory and collaborators from the University of Minnesota and Cleveland Clinic held their annual retreat at the University of Minnesota Landscape Arboretum in Chaska, MN on July 1st. This great day of scientific discussion and brainstorming ended with dinner and a park concert in Plymouth, MN!
Congratulations to Angela, Patrick, Jesse, and Divya, who presented their research projects at the Undergraduate Research Opportunities Program (UROP) symposium in April 2015!
The University of Minnesota hosted the American Society of Mechanical Engineers 4th Global Conference on NanoEngineering in Medicine and Biology in April 2015. Dave gave the keynote lecture in the "Cell-ECM interactions in mechanotransduction" section. Ghaidan and Louis both presented posters, and Louis' poster "Nanoscale measurements of microtubule assembly kinetics and tip structure" received Honorable Mention in the poster competition!
Graduate student Emily Tubman traveled to Oklahoma City, OK to present her poster "Transition-state model of kinetochore-microtubule attachments in budding yeast" at the Emerging Topics in Genome Instability Symposium in April 2015. Great work, Emily!
The Odde laboratory has entered the social media world! Follow @DavidOddeLab on Twitter to receive updates on ongoing research projects, conferences, publications, and more. https://twitter.com/davidoddelab
Dave, Brannon, and Ghaidan traveled to Baltimore, MD for the Biophysical Society meeting in February 2015. Ghaidan presented a poster titled "Finite Element Modeling of Cell Traction Force", and Brannon gave a talk titled "Molecular Recognition of Actin Turnover at the Leading Edge of Migrating Cells." Good job, everyone!
Benjamin Bangasser defended his thesis "Optimality in the nanomechanics of cell migration and adhesion" in September 2014, and is currently working in Minneapolis at Valspar as an Engineer III. Great job, Dr. Ben!
Dave, Brannon, and Brian traveled to the "Mathematics of the Cell: Integrating Genes, Biochemistry and Mechanics" meeting at the Banff International Research Station in September 2014.
Brian Castle defended his thesis “Multiscale modeling and analysis of microtubule self-assembly dynamics” in July (2014). Brian continues to work in the Odde lab as a post-doc. Well done, Dr. Castle!
Graduate student Louis Prahl received the NSF Graduate Fellowship in the spring of 2014. Keep up the good work, Louis!
An updated version of our microtubule tracking algorithm (TipTracker_v3) is now available for download here . The download package comes as a zip file that contains the tracking algorithm (two Matlab files) and a PDF support manual. This code update follows our recent chapter Quantitative analysis of microtubule self-assembly kinetics and tip structure in Methods in Enzymology, which contains additional information on code performance under various imaging conditions, and optimization procedures for potential user applications.
David Odde, Ph.D., IEM Cellular and Molecular Bioengineering Theme Co-Chair and Professor of Biomedical Engineering, was recently awarded a new R01 grant from the National Cancer Institute to develop an integrated modeling and microsystems approach to glioma invasion.
The $2.3M grant will involve researchers from Neurosurgery, the Masonic Cancer Center, the College of Science & Engineering and a collaboration with the Cleveland Clinic (Prof. Steve Rosenfeld, M.D., Ph.D.). A major goal will be to develop a "flight simulator" to simulate the mechanics of brain cancer cell migration in the brain, and use the simulator to identify possible therapeutic targets.
In this study, Prof. Odde and colleagues will be using a combination of advanced genetics, mouse models, and confocal light microscopy imaging to quantitatively measure glioma cell migration and force generation dynamics in live mouse brain slices. In parallel, they are developing advanced in vitro systems with controlled chemical and mechanical properties that more closely mimic normal and diseased brain tissue. Finally, these advanced experimental systems will be used to develop the "flight simulator" for the migrating cancer cells. This computer model will then be used to screen in silico for the most vulnerable nodes, and node combinations, in the mechanochemical network. Ultimately, they hope to find drug target combinations that strongly and selectively disable glioma cell migration, leading to new and more effective therapies.
Brian Castle's paper Brownian Dynamics of Subunit Addition-Loss Kinetics and Thermodynamics in Linear Polymer Assembly has been published in the current Biophysical Journal. In addition, Brian's image (shown at the left) was featured as the cover image of the current issue! Read more about the cover art here . Congratulations, Brian!
On the heels of his last publication, hot off the press is Ben's most recent paper Master Equation-Based Analysis of a Motor-Clutch Model for Cell Traction Force in CMBE. Way to go, Ben!
Becky Klank was awarded outstanding poster in the Cellular and Molecular Bioengineering Theme for her poster Biphasic Cell Migration and Progression of Malignant Gliomas. Well done, Becky!
Ben's paper Determinants of maximal force transmission in a motor-clutch model of cell traction in a compliant microenvironment was published in the Biophysical Journal in August 2013. Congratulations, Ben!
Former student Joseph Powers defended his Master's thesis entitled The Predicted Role of Stereospecificity in Crowding-Mediated Effects on Reversible Association in the summer of 2013. Joe then packed up his guitar and moved West to pursue a PhD in Bioengineering at the University of Washington. Lucky for the Huskies!
The Odde lab participated in Feed my Starving Children's food-packing event, which provides meals to malnourished children in over 70 countries around the world.
Former undergraduate researchers Alistair McIntyre and Ben Fuller graduated in the spring of 2013. Alistair is currently pursuing a MS in Bioengineering at the University of California, San Diego, and Ben is attending medical school at the University of Wisconsin, Madison. Thanks for your hard work and good luck in your endeavors!
The University of Minnesota Doctoral Dissertation Fellowship will fund Becky's final year of graduate studies at the University of Minnesota. She will be completing her work on novel imaging and modeling approaches for understanding glioma tumor progression. Congratulations, Becky!
Dave returned to MBL as a Physiology Course Faculty member in July 2013. Graduate students Louis Prahl and Emily Tubman traveled with him as teaching assistants. Over the course of the two week rotation, Physiology Course students developed and presented computational models of their research. In addition, course participants got to test their models using "bodystorming" with members of the Black Label Movement dance company.
The Odde lab held its first annual Odde Lab Retreat at the University of Minnesota Landscape Arboretum June 27, 2013. The lab used this time to discuss all current projects and brainstorm new ideas with collaborators from the University of Minnesota and the Cleveland Clinic.
Dave presented "If truth is beauty, can art be science?" with collaborator Carl Flink, Department of Theatre Arts & Dance at the TEDMED2013 Event at the Kennedy Center for Performing Arts in Washington, DC, on April 17, 2013.
Dave and UMN Department of Theatre Arts and Dance Professor Carl Flink's collaboration is making news in the scientific community. On the heels of their recent publication Science+Dance=Bodystorming (Trends in Cell Biology, 2012), Bodystorming: Dance Grooves Show How Molecules Move, published in Science NOW in November, describes the background and progression of the collaboration, as well as reactions from other scientists invited to join the pair to use bodystorming to test their own scientific hypotheses. Photo: William Cameron
Former graduate student Dominique Seetapun's paper Estimating the GTP cap size in vivo was published in Current Biology in September, 2012.
High school research participant Lucas Kramer participated in the Central Minnesota Science Fair where he presented his poster "The Effects of Microtubule Dynamics on Glioma Cell Motility: Does the Cell Length Dependent Model for Microtubule Activity in Neurons Apply to Human Glioma cells?" from work completed in the Odde lab under the mentorship of Brian Castle during the summer of 2012. He was selected to advance to the Intel International Science Fair in Phoenix in May.
Emily presented her poster "Kinesin-5 promotes disassembly of long kinetochore microtubules" at the Microtubule Cytoskeleton in Development and Disease meeting March 18-20, 2013 .
Becky presented her poster entitled "CD44-Mediated Motility in the Progression of Malignant Gliomas" at the PhysCell 2012 Meeting "Pierre-Gille de Gennes days on Physics of cells: from soft to living matter" in Hyeres, France held September 2-8, 2012.
Dave returned to the Marine Biological Laboratory in Woods Hole, MA as a Physiology Course Faculty member and brought Odde lab graduate students as his teaching assistants. In the two-week rotation, course students developed mathematical models of their home research topics and tested these models on Minneapolis-based dance company Black Label Movement.
In July, 2012, Ben gave a talk entitled "Optimality in a motor-clutch cell traction model" at the Signaling by Adhesion Receptors Gordon Research Seminar at Colby College in Waterville, ME. Well done, Ben!
With support from the NIH Neuorimaging Training Fellowhip, Becky will be using optical coherence tomography and two-photon imaging to study brain cancer motility. Congratulations, Becky!
With support from the UMN Graduate School IDF, Emily will collaborate with dancers, using "body-storming," to develop error correction models in mitosis during the 2012/2013 academic year. Congratulations, Emily!
The SPRBM and BMES are hosting the second inaugural Conference on Cellular and Molecular Bioengineering Jan. 3-7, 2012, at the Hilton Caribe Hotel and Resort in Puerto Rico.
Effective July 1, 2011, Dave joined the editorial board of the Biophysical Journal, the official journal of the Biophysical Society.
Dominique Seetapun, a recent graduate of the Odde lab, defended her thesis “Multiscale Microtubule Plus-End Dynamics in Neurons and Epithelial Cells” last May, 2011. Dominique is now a Senior R&D Scientist at Miromatrix Medical.. Congratulations, Dr. Seetapun!
Graduate student Ben Bangasser received the NSF Graduate Fellowship in the spring of 2011. Congratulations, Ben!